Relative In Vitro Protein Binding Characteristics of Diclofenac Sodium, Pantoprazole and Gatifloxacin With Human Serum And Plasma

Drug-plasma protein binding is one of the many factors which influence bioavailability of a drug. Efficacy, distribution and elimination of a drug can be affected by the reversible binding of the drug to plasma proteins, such as albumin and acid glycoprotein. The purpose of the study was to study the relative in vitro protein binding characteristics of various drugs like diclofenac sodium, pantoprazole and gatifloxacin in the presence of human plasma and serum proteins. Two ml solution of these drugs was mixed with 2 ml of serum and plasma separately and was allowed to dialyze through eggshell membrane and analyzed in a UVVisible spectrophotometer at respective absorption maxima wavelength. The result of the studies revealed that pentoprazole showed maximum binding with proteins followed by diclofenac sodium and gatifloxacin.